FIV_ELISA_Icon.jpgFeline Immunodeficiency Virus Antibody Test (ELISA)

Our Feline Immunodeficiency Virus (FIV) Antibody ELISA was developed twenty- years ago and has been used strictly as an in-house test in the intervening years because of a prohibitive exclusive patent held by IDEXX Corporation. That patent has expired so we are now free to market our test. The test is an Enzyme Linked Immunosorbent Assay (ELISA) that uses the Petaluma FIV strain which is absorbed to the plastic surface of Immulon Immunoassay Removawells. When a properly diluted cat serum is placed into the plastic well, the absorbed virus will capture any specific antibody which the cat has produced against the virus. The application of a second antibody which is directed against cat IgG and which is labeled with a Peroxidase Enzyme can then react with and bind to any cat antibody that is bound to the virus, which in-turn is bound to the plastic well. The resulting virus/antibody complex can be visualized by adding a Substrate to the well that can be degraded by the Peroxidase Enzyme resulting in a visual color change that is proportional and measurable to the amount of cat anti-virus antibody that is present in the cat serum. The color change is stopped by the addition of a weak acid, and the final product can be scanned on a plate reading spectrophotomer at 405nm. The results can be compared with positive and negative control serums and an accurate determination of the level of cat anti-virus antibody can be ascertained. The test requires only thirty-minutes to complete. Extensive comparison studies with Fluorescent Antibody and Western Blot analysis have been done and the results of those comparisons are available upon request.

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